dNMP, or deoxyribonucleoside monophosphate, is a building block of DNA. It consists of a deoxyribose sugar, a nitrogenous base (adenine, guanine, cytosine, or thymine), and a phosphate group. The nitrogenous base determines the specific nucleotide, and these nucleotides are paired with their complementary counterpart to form the rungs of the DNA ladder.
Initiation: DNA replication begins at specific locations in the genome called origins of replication. Proteins bind to these origins and unwind the DNA to create a replication fork.
Primer Synthesis: An RNA primer is synthesized by primase. This primer provides a starting point for DNA synthesis.
Elongation: DNA polymerase enzymes add dNTPs to the growing DNA strand. The enzyme reads the template strand and incorporates the complementary dNTP. For example, if the template strand has an adenine (A), the polymerase will add a thymine (T) dNTP to the new strand.
Formation of Phosphodiester Bonds: When a dNTP is added to the growing DNA strand, it loses two of its three phosphate groups (as pyrophosphate), and a phosphodiester bond is formed between the 3' hydroxyl group of the last nucleotide in the chain and the 5' phosphate group of the incoming dNTP.
Proofreading: DNA polymerases have proofreading activity that checks and corrects any mismatched bases to ensure high fidelity in DNA replication.
Termination: Once the entire DNA molecule has been replicated, the process concludes, and the replication machinery disassembles.
Our dNMP products boast exceptional purity, ensuring minimal contaminants that could interfere with experiments. Specifically optimized for DNA replication research, dNMP facilitates accurate strand elongation, providing reliable results for molecular biology studies.
Huaren Science’s dNMP is ideal for a range of applications, including the investigation of DNA damage repair, development of dNTP to dNMP conversion assays, and creation of synthetic DNA templates. The versatility of dNMP in DNA replication makes it indispensable for exploring replication mechanisms and advancing genomic research.
With scalable production capabilities, we cater to both small-scale researchers and industrial clients, ensuring consistent supply and premium quality. Backed by our expertise in nucleic acid technologies, Huaren Science provides comprehensive technical support to meet your research goals.